In situ hybridization and RT-PCR detection of Macrobrachium rosenbergii nodavirus in giant freshwater prawn (Macrobrachium rosenbergii) in Taiwan | Faculty Open Information System

In situ hybridization and RT-PCR detection of Macrobrachium rosenbergii nodavirus in giant freshwater prawn (Macrobrachium rosenbergii) in Taiwan

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題In situ hybridization and RT-PCR detection of Macrobrachium rosenbergii nodavirus in giant freshwater prawn (Macrobrachium rosenbergii) in Taiwan
出版類型SCI(Sciences Citation Index)
出版年度2006
AuthorsTsai Shinn Shyong, 蔡信雄
期刊Journal of Fish Diseases.29:665-671
出版日期2006 / 7
其他編號0000
中文摘要

Between January and March 2006,suspected outbreaks of white tail disease(WTD) were observed in plstlarvae and juveniles of M.rosenbergii in hatcheries and nursery ponds at Kaohsiung and Pingtung Counties in southern Taiwan. Pathognomonic lesions showed the presence of large oval and/or irregular basophilic,cytoplasmic inclusion bodies in the infected muscles and hepatopancreas. Reverse transcriptase polymerase chain reaction(RT-PCR)assay and in situ hybridization(ISH)revealed evidence of Macrobrachium rosenbergii nodavirus (MrNV)infection and failure reaction to extra small virus (XSV).The phylogenetic analysis indicated that,compared with their nucleotide sequences, there were very high identities among our six strains (99.5-100%),moderate with Caribbean and India strains (98.2-98.6),but slightly lower with Chinese strain (95.2%).This is the first confirmation of MrNV in giant freshwater prawns,via RT-PCR and ISH,in Taiwan

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