Monoclonal antibody-based ELISA for assessment of endpoint heating temperature of ground pork and beef

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Monoclonal antibody-based ELISA for assessment of endpoint heating temperature of ground pork and beef
出版類型SCI(Sciences Citation Index)
出版年度2002
AuthorsShyang-Chwen Sheu, 季蘊華, 張生, 陳福祺, & Shyang-Chwen Sheu 許祥純
開始頁1149
頁數5
出版日期2002 / 4
其他編號0000
中文摘要

An indirect enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody(MAb) 2F8 specific to a mammalian muscle protein was developed to evaluate the endpoint heating temperature (EPT) in ground pork and beef. The ELISA response of MAb 2F8 with meat extracts increased rapidly as the EPT increased from 60 to 80 °C
and leveled off above 80 °C. Results of immunoblot revealed that the 12 kD antigenic thermo-stable muscle protein, which is common in both pork and beef, can be used as an EPT indicator for heat-processed pork and beef based on changes in the ratio of this protein to total soluble proteins in the meat extract which occurs at the EPT. Keywords: endpoint temperature, meat, thermo-stable muscle protein, monoclonal antibody, ELISA

期刊名稱J. Food Sci.
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