Detection of Psuedorabies Virus in Tissues Using In Situ Hybridizaiton and Ploymerase Chain Reaction Techniques

in Domestic Journal (NSC excellent journals), 國內期刊(國科會優良期刊)
標題Detection of Psuedorabies Virus in Tissues Using In Situ Hybridizaiton and Ploymerase Chain Reaction Techniques
出版類型國內期刊(國科會優良期刊)
出版年度2001
AuthorsHung-Jen Liu, 林茂勇
期刊Journal of the Chinese Society of Veterinary Science, 2001, 27(1): 19-26
出版日期2001 / 1
其他編號0000
中文摘要

An insitu hybridization (ISH) and in situ polymerase chain reaction (in situ PCR) tests with a digoxigenin (DIG)-labeled DNA probe were used to detect the latency of pseudorabies virus (PRV) DNa in formalin-fixed, paraffin-embedded pig tissues. Tissues were collected 3 months following inoculation with the Pt strain of PRV. To confirm the specificity of ISH and in situ PCR. PCR was used to amplify the IE and gE genes of the PRV PT strain. In situ methods are more rapid and accurate for the diagnosis of latent PRV infections. The senitivity of these two techniques was compared. The in situ PCR test was found to be more sensitvie than ISH and allowed the rapid, sensitive, and specific detection of latent PRV infections.

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