Metschnikowia bicuspidata dominates in Taiwanese cold-weather yeast infections of Macrobrachium rosenbergii

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Metschnikowia bicuspidata dominates in Taiwanese cold-weather yeast infections of Macrobrachium rosenbergii
出版類型SCI(Sciences Citation Index)
出版年度2007
AuthorsShih-Chu Chen, 陳石柱
開始頁191
頁數8
出版日期2007 / 5
其他編號0000
中文摘要

ABSTRACT: At water temperatures below 17°C, yeast infections often occurred in 6 to 11 mo old giant
freshwater prawn Macrobrachium rosenbergii (8 to 12 cm) in Taiwan from May 2001 to December 2003,
with a cumulative mortality of 20 to 95%. Gross signs of disease included milky hemolymph, a yellow exoskeleton,
opaque muscles, and a swollen hepatopancreas (HP). Histopathology included marked
edema and extensive necrotic lesions associated with large numbers of yeast aggregates and inflammation
within the muscles, HP, and other internal organs such as the heart, ovary, and intestine. Yeast cell
numbers isolated from various tissues ranged from 4.5 × 108 to 9.0 × 109 colony forming units (CFU) per
100 mg. From diseased prawns from 12 affected farms, the presence of Metschnikowia bicuspidata
(98.4% prevalence), Saccharomyces cerevisiae (0.8% prevalence), and Candida albicans (0.8% prevalence)
was confirmed by biochemical tests and sequencing of PCR products from the D1/D2 domain
of 26S rDNA. Experimental infection with these isolates caused gross signs and histopathological
changes similar to those observed in naturally infected prawns, and lethal doses (LD50) were 3.8 × 103,
2.0 × 103, and 4.3 × 103 CFU prawn–1, respectively. Although the results of this study revealed that M. bicuspidata
may be the major cause of yeast infections in the giant freshwater prawns in Taiwan, this is the
first time that S. cerevisiae and C. albicans are also reported as pathogens.

期刊名稱DISEASES OF AQUATIC ORGANISMS
校址:912 屏東縣內埔鄉學府路1號 總機:886-8-7703202 傳真:886-8-7740165 系統開發統維護單位:國立屏東科技大學 電算中心 版權所有