The molecular evolution of Momordica charantia L. the Ribosome-inactivating protein gene family.

in Domestic Conference (poster summary), 國內研討會(摘要海報發表)
標題The molecular evolution of Momordica charantia L. the Ribosome-inactivating protein gene family.
出版類型國內研討會(摘要海報發表)
出版年度2007
AuthorsYu-Chung Chiang, 江友中
會議名稱中華植物學會第二十五屆第二次會員大會暨植物科學與生質能源前瞻學術研討會
出版日期Nov 24 2007 12:0
會議地點Taipei
其他編號0000
中文摘要

Ribosome-inactivating proteins (RIPs) have adenine glycosylase activity. It can inhibit protein synthesis and protect plants to against pathogens. In recent research, scientists study the function of RIPs and develop the new medicine to anti-tumor and anti-HIV. The molecular variations of RIPs gene family, however, are still unclear. In this study, we use bitter melon (Momordica charantia L.) as a model system to define the molecular variations and evolutions in the RIPs gene family. The evolutionary diversification of genomes and genetic systems is driven in part by duplication events. To investigate the genetic diversity and possible molecular evolutionary hypothesis within and among the wild, hybridization, and cultivated varieties of bitter melons, samples were analyzed using PCR to sequence loci from the RIPs gene (named copy1-3) and neutral chloroplast genome. Nucleotide diversity and divergence were detected 0.01045 (θ) and 0.00324 (π) in copy 1, 0.01421 and 0.00373 in copy 2, and 0.01174 and 0.00290 in copy 3. Test of selection and neutrality (Tajima’s D) were performed to assess the evolutionary processes influencing each gene region and indicated significant values for copy 1 (D = -2.371, p<0.01), copy 2 (D = -2.478, p<0.01) and copy 3 (D = -2.481, p<0.01), but not for the cpDNA atpB-rbcL spacer for bitter melon. Significant values can be indicative of selective sweep or may be selective force in the Ribosome-inactivating proteins gene family.

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