Cloning and nucleotide sequencing of the second internal 3 transcribed spacer of ribosomal DNA for three species of 4 eimeria from chickens in Taiwan | Faculty Open Information System

Cloning and nucleotide sequencing of the second internal 3 transcribed spacer of ribosomal DNA for three species of 4 eimeria from chickens in Taiwan

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Cloning and nucleotide sequencing of the second internal 3 transcribed spacer of ribosomal DNA for three species of 4 eimeria from chickens in Taiwan
出版類型SCI(Sciences Citation Index)
出版年度2008
AuthorsYi-Yang Lien, 連一洋, Shyang-Chwen Sheu 許祥純, & 蘇弘益
開始頁184
頁數5
出版日期2007 / 1
其他編號0000
中文摘要

Coccidiosis of chickens caused by protozoan parasites of the genus Eimeria (Coccidia: Eimeriidae) is an enteric disease that
results in great economic losses throughout the world, including Taiwan. Using polymerase chain reaction (PCR) with primers specific
for the second internal transcribed spacer (ITS-2) of ribosomal DNA (rDNA), three species of Eimeria, E. tenella, E. maxima,
and E. acervulina have been successfully characterised from chickens in Taiwan. The sizes of PCR products from various isolates
representing these three species were between 370 and 580 base pairs (bp). After cloning and sequencing of the PCR products, high
nucleotide sequence identity (96.8–100%) was observed within a species. In addition, ITS-2 nucleotide sequences for E. tenella had
higher homology (98.5–99.3%) than E. maxima (81.6–96.5%) when compared with appropriate sequences deposited in GenBank. To
our knowledge, this is the first report of a 412-bp ITS-2 sequence for E. acervulina from chickens.

期刊名稱The Veterinary Journal
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