A second proPO present in white shrimp Litopenaeus vannamei and expression of the proPO during a Vibrio alginolyticus injection, moult stage, and oral sodium alginate ingestion

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題A second proPO present in white shrimp Litopenaeus vannamei and expression of the proPO during a Vibrio alginolyticus injection, moult stage, and oral sodium alginate ingestion
出版類型SCI(Sciences Citation Index)
出版年度2009
AuthorsMaw-Sheng Yeh, 葉茂盛, Chun-Hung Liu 劉俊宏, Ching-Ming Kuo 郭欽明, & Winton Cheng 鄭文騰
開始頁49
頁數6
出版日期2009 / 1
其他編號NSC-96-2313-B-020-009
中文摘要

Prophenoloxidase (proPO) is a melanin-synthesising enzyme that plays important roles in immune responses by crustaceans. Previously, we cloned and characterized proPO-I from white shrimp, Litopenaeus vannamei. In the present study, a novel prophenoloxidase-II (proPO-II) cDNA was also cloned from haemocytes of L. vannamei using oligonucleotide primers and reverse-transcriptase polymerase chain reaction (RT-PCR). Both 30- and 50-regions were isolated by the rapid amplification of complementary (c)DNA end (RACE) method. The 2504-bp cDNA contained an open reading frame (ORF) of 2073 bp, an 84-bp 50-untranslated region, and a 347-bp 30-untranslated region containing the poly A tail. The molecular mass of the deduced amino acid sequence (691 amino acids) was 78.8 kDa with an estimated pI of 6.07. It contains two putative tyrosinase copper-binding motifs and a conserved C-terminal region common to all known proPOs. Comparisons of the amino acid sequences showed that white shrimp proPO-II is more closely related to the proPO of other penaeids than to that of crayfish, lobsters, crab, or a freshwater prawn, and is the ancestor type of known penaeid proPOs. proPO-I and proPO-II messenger
(m)RNAs of shrimp were located on different loci, and were constitutively expressed mainly in haemocytes. The transcriptional regulation of these two proPOs in shrimp at different molt stages, those administered dietary sodium alginate, and those challenged with Vibrio alginolyticus were surveyed. The results showed that the proPOs may be directly involved in the acute-phase immune defence, and
proPO-II may contribute earlier to immune defence in shrimp injected with V. alginolyticus, and it may be regulated by ecdysone. However, a similar effect was found by stimulating proPO-I and proPO-II mRNA expression in shrimp fed a sodium alginate-containing diet. Results of this study provide a basis for developing a comprehensive understanding of expression/function relationships of individual proPOs in shrimp.

期刊名稱Fish Shellfish Immunol.
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