Utilizing a cell concentration monitoring model for establishing automatic control and optimization strategies for fed-bath fermentation of yeast.

in Domestic Conference (poster paper), 國內研討會(全文海報發表)
標題Utilizing a cell concentration monitoring model for establishing automatic control and optimization strategies for fed-bath fermentation of yeast.
出版類型國內研討會(全文海報發表)
出版年度2007
AuthorsJia-Hsin Guo, 郭嘉信, & 陳彥竹
會議名稱台灣食品科技學會第37次年會
出版日期Dec 7 2007 12:0
會議地點大葉大學活動中心
其他編號0000
中文摘要

Saccharomyces cerevisiae is a popular eukaryote platform for expressing simple heterologous proteins. Growing S. cerevisiae to high densities has been frequently studied to achieve maximum productivity. A corrected feed-forward strategy was developed for this purpose. The strategy incorporates a method for estimation of specific growth rate as a performance indicator of the fed-batch culture. The estimation is based on the measurement of the maximum substrate uptake rate using residual glucose concentration data and mass balances. It allows us to track changes in the specific growth rate to compensate for process disturbances. The strategy may be applied to fed-batch culture of S. cerevisiae to maximize the volumetric productivity of cell mass. For a such study, a kinetic model was founded and used here as follows: VX = V0X0exp(μt); F = [V0X0exp(μt)] ÷ [YX/S (Si – S)], meanwhile V and V0 were fermentation volume and initial volume, respectively; μ was specific growth rate; X and X0 were yeast concentration and initial concentration, respectively; t was fermentation time; F was feeding rate; YX/S was yeast biomass yield on glucose; Si and S were glucose concentrations of feeding medium and fermentation broth, respectively. According to our primary results, we have found that S. cerevisiae, in YM broth, can perform a better biomass yield on glucose when glucose concentration reaches 5% (w/v). Under this condition, we can set up a regulating feeding rate based on the preceding kinetic model to maintain better specific growth rates of S. cerevisiae within 0.038-0.064 hr-1 and good yields varied from 0.066-0.085 g of cell mass (dry wt) per gram of glucose.

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