Hepatitis B virus X protein activates a survival signaling by linking Src to Phosphatidylinositol 3-kinase

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Hepatitis B virus X protein activates a survival signaling by linking Src to Phosphatidylinositol 3-kinase
出版類型SCI(Sciences Citation Index)
出版年度2003
AuthorsWen-Ling Shih, 施玟玲
開始頁31807
頁數6
出版日期2003 / 6
其他編號0000
中文摘要

We have previously shown that transactivation-proficient
hepatitis virus B X protein (HBx) protects Hep 3B
cells from transforming growth factor- (TGF-)-induced
apoptosis via activation of the phosphatidylinositol
3-kinase (PI 3-kinase)/Akt signaling pathway. This
work further investigated how HBx activates PI 3-kinase.
Src activity was elevated in Hep 3B cells following
expression of transactivation-proficient HBx or HBx-
GFP fusion proteins. The Src family kinase inhibitor
PP2 and C-terminal Src kinase (Csk) both alleviated
HBx-mediated PI 3-kinase activation and protection
from TGF--induced apoptosis. Therefore, HBx activated
a survival signal by linking Src to PI 3-kinase.
Systemic subcellular fractionation and membrane flotation
assays indicated that 1.5% of ectopically expressed
HBxGFP was associated with periplasmic membrane
where Src was located. However, neither nucleustargeted
nor periplasmic membrane-targeted HBxGFP
was able to upregulate Src activity or to augment PI
3-kinase survival signaling pathway.

期刊名稱The Journal of Biological Chemistry
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