Avian reovirus sigmaC protein induces apoptosis in cultured cells.

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Avian reovirus sigmaC protein induces apoptosis in cultured cells.
出版類型SCI(Sciences Citation Index)
AuthorsWen-Ling Shih, 施玟玲, Liao Ming-Huei 廖明輝, & Hung-Jen Liu 劉宏仁
出版日期2004 / 3

The avian reovirus (ARV) infection is associated with various disease conditions in poultry. However, the pathogenesis mechanisms are
poorly characterized. In the present study, we clearly demonstrated that the jC of ARV S1133 strain induced apoptosis in both BHK-21 and
Vero cells. Five kinds of assays for apoptosis were used in analyzing ARV-infected BHK-21 and Vero cells: (1) assay for DNA ladders, (2)
ELISA detection of cytoplasmic histone-associated DNA fragments, (3) nuclear staining with acridine orange, (4)Western blot, Northern blot,
and immunofluorescent assay (IFA), and (5) flow cytometric analysis. The jC protein of ARV could elicit apoptosis occurring in a dose- and
time-dependent manner. The current results further our understanding of the function of jC in cultured cells and suggest that jC is a viralencoded
apoptin and possesses apoptosis-inducing ability. Furthermore, deletion analysis of the ARV jC protein suggests that the carboxylterminus
of jC is important in mediating jC-induced apoptosis because its deletion abolished the induction of apoptosis.
D 2004 Elsevier Inc. All rights reserved.

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