Rapid characterization of avian reoviruses using phylogenetic analysis, reverse transcription-polymerase chain reaction and restriction enzyme fragment length polymorphism.

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Rapid characterization of avian reoviruses using phylogenetic analysis, reverse transcription-polymerase chain reaction and restriction enzyme fragment length polymorphism.
出版類型SCI(Sciences Citation Index)
出版年度2004
AuthorsWen-Ling Shih, 施玟玲, & Hung-Jen Liu 劉宏仁
開始頁171
頁數9
出版日期2004 / 4
其他編號0000
中文摘要

A reverse transcription-polymerase chain reaction is described, which amplified the full-length sC-encoding and
sNS-encoding genes of avian reovirus (ARV). DNA fragments of 1022 and 1152 base pairs were amplified
among ARV isolates, respectively, indicating that there were no apparent deletions or insertions in these regions.
Fragments amplified from vaccine strains and field isolates were digested with five different restriction enzymes
Bcn I, Hae III, Taq I, Dde I, and Hinc II, respectively. Restriction fragment profiles observed on
polyacrylamide gels showed heterogeneity between vaccine and Taiwanese isolates. All ARV isolates tested
showed different restriction enzyme cleavage patterns and could be clearly distinguished. The strain-typing
based on the cleavage sites in the sC-encoding gene of ARV showed that viruses could be classified into four
distinct groups. A phylogenetic tree based on the nucleotide sequences of the sC-encoding gene revealed that
Taiwanese ARV isolates were classified into four distinct groups, indicating that the genotyping is consistent
with typing based on restriction enzyme fragment length polymorphism of the sC-encoding gene of ARV. The
results suggested that polymerase chain reaction followed by restriction enzyme analysis provided a simple and
rapid approach for characterization of ARV isolates. Also, it is possible to determine whether a new variant
strain has been introduced into a flock or a given virus strain has spread from one flock to another.

期刊名稱Avian Pathology
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