Avian Reovirus activates a novel proapoptotic signal by linking Src to p53 | Faculty Open Information System

Avian Reovirus activates a novel proapoptotic signal by linking Src to p53

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Avian Reovirus activates a novel proapoptotic signal by linking Src to p53
出版類型SCI(Sciences Citation Index)
出版年度2006
AuthorsWen-Ling Shih, 施玟玲, & Hung-Jen Liu 劉宏仁
開始頁2179
頁數14
出版日期2006 / 12
其他編號0000
中文摘要

Abstract We have previously shown that avian reovirus
(ARV) S1133 and its structural protein σC cause apoptosis
in cultured Vero cells through an unknown intracellular
signaling pathway. This work investigates how ARV S1133
induces proapoptotic signals. Upon ARV S1133 infection
and subsequent apoptosis, levels of p53 mRNA and protein,
and p53 serine-46 and serine-392 phosphorylation increased.
In addition, p53-driven reporter activity and levels
of the p53-induced apoptotic protein bax were increased,
and Src tyrosine-418 phosphorylation was elevated. UVinactivated
virus failed to activate Src, p53 or induce apoptosis.
Over-expression of dominant negative p53, or treatment
with tyrosine kinase inhibitor genistein protected cells from
ARV S1133-induced apoptosis. Inhibition of Src by overexpression
of C-terminal Src kinase (Csk) or treatment with
Src family tyrosine kinase inhibitor SU-6656 diminished the
ARV S1133-induced p53 expression, activation, and apoptosis.
Over-expression of σC resulted in the upregulation of p53, p53 serine-46 phosphorylation, p53-driven reporter activity
and accumulation of bax. σC expression during ARV
S1133 infection was concomitant with the onset of apoptosis.
These studies provide strong evidence that the viral gene
expression is required for ARV S1133 to initiate a proapoptotic
signal via Src to p53. In addition, σC was able to utilize
a p53-dependent pathway to elicit apoptosis.

期刊名稱Apoptosis
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