Development and characterization of monoclonal antibodies against avian reovirus sigmaC protein and their application in detection of ARV isolates.

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Development and characterization of monoclonal antibodies against avian reovirus sigmaC protein and their application in detection of ARV isolates.
出版類型SCI(Sciences Citation Index)
出版年度2006
AuthorsWen-Ling Shih, 施玟玲, & Hung-Jen Liu 劉宏仁
開始頁320
頁數6
出版日期2006 / 8
其他編號0000
中文摘要

Avian reovirus (ARV) is a non-enveloped virus with a segmented double-stranded RNA genome surrounded
by a double icosahedral capsid shell. ARVs are associated with viral arthritis, immunosuppression, and
enteric diseases in poultry. The sC protein was involved in induction of apoptosis and neutralization
antibody. In the present study, sC-His protein was expressed in Sf9 insect cells and purified by immobilized
metal affinity chromatography. Eight monoclonal antibodies (mAbs) against sC-His and three mAbs
against His were screened from hybridoma cells produced by fusion of splenocytes from immunized mice
with NS1 myeloma cells. Among the eight mAbs against sC protein, all belonged to the IgG isotype except
three for IgM. It was discovered that all anti-His mAbs were mixtures of IgG and IgM isotypes. mAbs
reacted with sC-His protein in a conformation-independent manner based on dot blot and western blotting
assays. The competitive binding assay indicated that all mAbs recognized the same epitope on sC protein
that was conserved in different isolates. Compared with the commercial anti-ARV S1133 polyclonal
antibody, mAb (D15) had universal reactivity to all serotypes or genotypes of ARVs tested. This monoclonal
antibody may therefore be useful for the development of an antigen-capture enzyme-linked immunosorbent
assay for rapid detection of field isolates.

期刊名稱Avian Pathology
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