Optimization of lactic acid production by immobilized Lactococcus lactis IO-1

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Optimization of lactic acid production by immobilized Lactococcus lactis IO-1
出版類型SCI(Sciences Citation Index)
出版年度2007
AuthorsSarote Sirisansaneeyakul, S. S., Sarote Sirisansaneeyakul S. S., Sarote Sirisansaneeyakul S. S., Tiyaporn Luangpipat T. L., Tiyaporn Luangpipat T. L., Tiyaporn Luangpipat T. L., Wirat Vanichsriratuna W. V., Wirat Vanichsriratuna W. V., Wirat Vanichsriratuna W. V., Thongchai Srinophakun T.;ngchai S.;phakun, Thongchai Srinophakun T.;ngchai S.;phakun, Thongchai Srinophakun T.;ngchai S.;phakun, Ho-Hsien Chen 陳和賢, Y. C., Y. C., & Y. C.
開始頁381
頁數10
出版日期2007 / 1
其他編號0000
中文摘要

Production of lactic acid from glucose by
immobilized cells of Lactococcus lactis IO-1 was
investigated using cells that had been immobilized by
either entrapment in beads of alginate or encapsulation
in microcapsules of alginate membrane. The fermentation
process was optimized in shake flasks using the
Taguchi method and then further assessed in a production
bioreactor. The bioreactor consisted of a
packed bed of immobilized cells and its operation involved
recycling of the broth through the bed. Both
batch and continuous modes of operation of the reactor
were investigated. Microencapsulation proved to be
the better method of immobilization. For microencapsulated
cells at immobilized cell concentration of
5.3 g l–1, the optimal production medium had the following
initial concentrations of nutrients (g l–1): glucose
45, yeast extract 10, beef extract 10, peptone 7.5
and calcium chloride 10 at an initial pH of 6.85. Under
these conditions, at 37 C, the volumetric productivity
of lactic acid in shake flasks was 1.8 g l–1 h–1. Use of a
packed bed of encapsulated cells with recycle of the
broth through the bed, increased the volumetric productivity
to 4.5 g l–1 h–1. The packed bed could be used
in repeated batch runs to produce lactic acid.

期刊名稱J Ind Microbiol Biotechnol
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