In Vitro Cellular Immune Responses to Recombinant Antigens of Mycobacterium avium subsp. paratuberculosis

in Social Sciences Citation Index(SSCI), 社會科學引用文獻索引資料庫(SSCI)
標題In Vitro Cellular Immune Responses to Recombinant Antigens of Mycobacterium avium subsp. paratuberculosis
出版類型SSCI(Social Sciences Citation Index)
出版年度2005
AuthorsSung Jae Shin, S. J. S., Chao-Fu Chang C. - F. C., CHING-DONG CHANG 張清棟, Sean P. McDonough S. P. M. D., Belinda Thompson B. T., Han Sang Yoo H. S. Y., & Yung-Fu Chang Y. - F. C.
期刊感染與免疫
開始頁5074
頁數11
出版日期2005 / 1
其他編號0000
中文摘要

Five recombinant antigens (Ags; 85A, 85B, 85C, superoxide dismutase [SOD], and 35-kDa protein) were purified from Mycobacterium avium subsp. paratuberculosis and evaluated for their ability to stimulate peripheral blood mononuclear cells (PMBCs) from fecal-culture-positive cows (low and medium shedders) and culture-negative healthy cows. Recombinant Ags 85A, 85B, and 85C induced significant lymphocyte proliferation as well as the production of gamma interferon (IFN- ), interleukin-2 (IL-2), IL-12, and tumor necrosis factor alpha (TNF- ), but not IL-4, from low and medium shedders. The 85 antigen complex did not stimulate PMBC proliferation from culture-negative healthy cows. The 35-kDa protein also induced significant lymphocyte proliferation as well as the production of IFN- and IL-4 from low and medium shedders. CD4 T cells and CD25 (IL-2R) T cells were stimulated the most by 85A and 85B, while the 35-kDa protein primarily stimulated CD21 B cells involved in humoral immune responses. Interestingly, SOD was less immunostimulatory than other antigens but strongly induced T cells, which are thought to be important in the early stages of infection, such as pathogen entry. These data provide important insight into how improved vaccines against mycobacterial infections might be constructed.

期刊名稱INFECTION AND IMMUNITY
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