Cellular effects in host cells induced by recombinant VP1 (rVP1) of foot-and-mouth disease virus (FMDV).

in International Symposium (poster paper), 國際研討會(全文海報發表)
標題Cellular effects in host cells induced by recombinant VP1 (rVP1) of foot-and-mouth disease virus (FMDV).
出版類型國際研討會(全文海報發表)
出版年度2006
AuthorsChung-Da Yang, 楊忠達, Jei-Ming Peng 彭瑞銘, & Shu-Mei Liang 梁淑美
出版日期Jan 5 2006 12:0
會議地點Buena Vista Palace Resort and Spa, Orlando, FL
其他編號0000
中文摘要

Cellular effects in host cells induced by recombinant VP1 (rVP1) of foot-and-mouth disease virus (FMDV)

Chung-Da Yang, Jei-Ming Peng, Shu-Mei Liang
Institute of BioAgricultural Sciences, Academia Sinica, Taipei, Taiwan

Abstract
Foot-and-moth disease virus (FMDV) is an etiological agent of foot-and-moth disease (FMD). Capsid protein VP1 of FMDV contains a highly conserved Arg-Gly-Asp (RGD) tripeptide, which binds to integrins and facilitates the viral internalization into target cells. We cloned and purified the recombinant VP1 (rVP1) in an aqueous form previously. In this study, we investigated the cellular effects induced by rVP1 in virus permissive cell, BHK-21. We found that the recombinant VP1 (rVP1) of FMDV induced apoptotic signals in BHK-21 cells after binding to integrins. In addition, we also observed that rVP1 reduced the expression of toll-like receptor 9 (TLR9), an immune sensor that recognized bacterial and virus DNA. The reduction of TLR9 expression by rVP1 was at both translational and transcriptional levels as shown by Western blot and RT-PCR analysis. These results showed that rVP1 may reduce host defensive response to invading pathogens and trigger apoptosis in host cells.

Keywords: FMD, FMDV, rVP1, apoptosis

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