Protective immunity against Toxoplasma gondii in mice induced by SAG2 internal image of anti-idiotype antibody.

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Protective immunity against Toxoplasma gondii in mice induced by SAG2 internal image of anti-idiotype antibody.
出版類型SCI(Sciences Citation Index)
出版年度2003
AuthorsChung-Da Yang, 楊忠達, Gan-Nan Chang 張甘楠, & David Chao 趙大衛
開始頁452
頁數5
出版日期2003 / 12
其他編號0000
中文摘要

A monoclonal antibody TGCM5 (isotype G, subclass 1, kappa light chain) against SAG2, a major surface antigen of Toxoplasma gondii tachyzoites, was produced in quantity and its Fab-containing idiotype (Id) was injected into rabbits for production of rabbit anti-Id sera. The rabbit anti-Id IgG (aId-IgG) with a SAG2 internal image was obtained by purification with a protein A column. Significant lymphocyte proliferation induced by tachyzoite lysate antigen was observed in BALB/c mice that received two injections of aId-IgG, but not in non-immunized control mice. Large amounts of gamma interferon were detected in supernatants of splenocyte cultures prepared from mice immunized with aId-IgG. Of the mice immunized with aId-IgG, 75-87.5% survived at least 28 days after a lethal challenge of 1 x 103 live tachyzoites, which killed all non-immunized control mice within 10 days. The results showed that the SAG2 internal image presented by aId-IgG indeed elicited a protection against the infection of T. gondii in mice.

期刊名稱Parasitology Research
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