Effects and optimization of thermal parameters on freezing injury in Fadu nasopharyngeal carcinoma cells

in International Symposium (poster summary), 國際研討會(摘要海報發表)
標題Effects and optimization of thermal parameters on freezing injury in Fadu nasopharyngeal carcinoma cells
出版類型國際研討會(摘要海報發表)
出版年度2008
AuthorsK. L. Fan, 范凱隆, Chien Chih Hou 侯建志, Wei Te Wu(Benny) 吳瑋特, M. -J. Tseng 曾銘仁, & Wen-Hsin Hsieh 謝文馨
出版日期Jul 23 2008 12:0
會議地點Charlotte, North Carolina (USA)
其他編號0000
中文摘要

This poster shows an experim ental invest igation of the effects of, and the optimization of, thermal control factors for freezing injury to FaDu nasopharyngeal carcinoma cells using the Taguchi method. Freeze/thaw experim ents based on the L
(2
8
7
) two-level orthogonal array of the Taguchi method were conducted. Analysis of variance on the experimental data was performed to determine the contributio n of each thermal control factor and the optimal test conditions for maximum cell injury and experimental repeatability were determined. The results show that the holding time, end temperature, thawing rate-holding time interact ion, and thawing rate (listed in the order of contribution) are the statistically signi?cant factors affecting cell viability, and cooling rate dominated the experim ental repeatability of this study. The cell viability was found to decrease with decrease in the thawing rate and end temperature and with increase in the holding time and cooling rate. The repeatability of the experiment increased with decreasing cooling rate. The optimal test condition was veri?ed by experiment.

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