The inhibition effect of 2,3,7,8-tetrachlorinated dibenzo-p-dioxin -induced aryl hydrocarbon receptor activation in human hepatoma cells with the treatment of cadmium chloride

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題The inhibition effect of 2,3,7,8-tetrachlorinated dibenzo-p-dioxin -induced aryl hydrocarbon receptor activation in human hepatoma cells with the treatment of cadmium chloride
出版類型SCI(Sciences Citation Index)
出版年度2009
AuthorsHow-Ran Chao/Hao-Jan Chao, 趙浩然, Tsui-Chun Tsou 鄒粹軍, Hung-Ta Chen 陳宏達, Eddy Essen Chang 張一旋, Feng-Yuan Tsai 蔡?源, Ding-Yan Lin 林鼎彥, Fu-An Chen 陳福安, & Ya-Fen Wang 王雅玢
開始頁351
頁數5
出版日期2011 / 1
其他編號0000
中文摘要

Polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs), considered as endocrine disruptors, tend to accumulate in fatty tissues. Dioxin-responsive element chemical activated luciferase gene expression assay (DRE-luciferase assay) has been recognized as a semi-quantitative method for screening dioxins for its fast and low-cost as compared with HRGC/HRMS. However, some problems with the bioassay, including specificity, detection variation resulted from different cleanup strategies, and uncertainty of false-negative or false-positive results, remain to be overcome. Cadmium is a prevalent environmental contaminant around the world. This study was aimed to examine the effects of cadmium on the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced activation of aryl hydrocarbon receptor (AhR)-mediated gene expression in human hepatoma cells (Huh7-DRE-Luc cells and Huh7 cells). Ethoxyresorufin-O-deethylase (EROD) and DRE-luciferase assaywere employed to determine the enzyme activity of cytochrome P450 1A1 (CYP1A1) and activation of AhR, respectively. The results showed that Cd2+ levels significantly inhibited the induction of TCDD-induced CYP1A1 and DRE luciferase activation in hepatoma cells. The 50% inhibited concentrations (IC50) of CdCl2 were 0.414M(95% confidence interval (C.I.): 0.230–0.602M) in Huh7-DRE-Luc cells and 23.2M (95% C.I.: 21.7–25.4M) in Huh7 cells. Accordingly, prevention of interference with non-dioxin-like compounds in a DRE-luciferase assay is of great importance in an extensive cleanup procedure.

期刊名稱Journal of Hazardous Materials
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