On Chip Particle Differentiation Utilizing Forward Scattering Light and Fluorescence Light with Passive Focusing

in International Symposium (oral presentation paper), 國際研討會(全文口頭發表)
標題On Chip Particle Differentiation Utilizing Forward Scattering Light and Fluorescence Light with Passive Focusing
出版類型國際研討會(全文口頭發表)
出版年度2010
AuthorsYao-Nan Wang, 王耀男, Jik Chang Leong 梁智創, CHIN-LUNG CHANG 張金龍, Tai 戴昌賢, Tsai Chien-Hsiung 蔡建雄, Fu Lung-Ming 傅龍明, & miao jr-ming 苗志銘
出版日期Dec 18 2009 12:0
會議地點上海
其他編號0000
中文摘要

In this paper, a microflow cytometer for particles/cells
passive focusing, fluorescence detection, and sizing is
demonstrated with a convergent geometry of 50 μm detection
gate channel and a pair of external optic fibers on polydimethylsiloxane
(PDMS) microchip. A laser (635 nm) is used
for fluorescent excitation. The amplitudes of the backward
fluorescence signal and forward scattered light signal indicate
the corresponding fluorescent intensity and size of the detected
fluorescent particles and labeled white blood cells, respectively.
In the experiment, the uniform fluorescent light intensity was
measured by focusing the fluorescent particles in a
200×50×30μm detection gate channel. However, the emitted
fluorescence and forward scattered light intensity of different
size particles/cells are also measured simultaneously by
external optic fibers.

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