Direct Lipase Catalyzed Lipophilization of Chlorogenic Acid from Coffee Pulp in Supercritical Carbon Dioxide

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Direct Lipase Catalyzed Lipophilization of Chlorogenic Acid from Coffee Pulp in Supercritical Carbon Dioxide
出版類型SCI(Sciences Citation Index)
出版年度2009
AuthorsCarlos E. Hernandez, 卡洛斯, Chang 張誌益, Ho-Hsien Chen 陳和賢, & Tzou-Chi Huang 黃卓治
開始頁359
頁數6
出版日期2009 / 3
其他編號0000
中文摘要

The direct esterification of chlorogenic acid (5-CGA) by immobilized Candida antarctica lipase B (Novozym 435) in supercritical CO2/t-butanol has been studied. The Taguchi approach was applied to evaluate the effects of temperature (35–55 °C), pressure (150–250 bar), t-butanol (2–10%, v/v), and the enzyme amount (10–30 mg/ml), on the ester concentration and overall conversion. Optimum reaction conditions were established at: 150 bar, 55 °C, 10% t-butanol (v/v), 20 mg/ml of lipase. Addition of 20 mg/ml of molecular sieves (3 A) was also necessary to minimize the inhibiting effect of the increasing water concentration. Maximum conversions reached 77, 82 and 85% in 25 h using geraniol, pentanol and heptanol as aliphatic chain donors, respectively. HPLC analysis demonstrated the selective esterification of 5-CGA from a coffee pulp aqueous-methanolic extract, which reached a conversion to heptyl ester of 65% at the optimal condition. The supercritical CO2 selectivity towards the esterified product was the working principle of this study, by which minimized interphase transport limitations and enhanced mass-transfer phenomena substantially improved the reaction kinetics.

期刊名稱Industrial Crops and Products
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