Neutrophils as one of the major haptoglobin sources in mastitis affected milk

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Neutrophils as one of the major haptoglobin sources in mastitis affected milk
出版類型SCI(Sciences Citation Index)
AuthorsI-Hsiang LAI, I. - H. L. A. I., Jung Hsu TSAO J. H. T. S. A. O., Yi Ping LU Y. P. L. U., Jai-Wei Lee 李嘉偉, Xin ZHAO X. Z. H. A. O., Feng Lin CHIEN F. L. C. H. I. E. N., & Simon J.T. MAO S. J. T. M. A. O.
出版日期2009 / 5

The antioxidant haptoglobin (Hp) is an acute-phase protein responsive to infectious and
inflammatory diseases. Hp and somatic cell counts (SCC) are sharply elevated in bovine milk
following intramammary administration of endotoxin or bacteria. However, the sources of milk Hp
responsible for such increases are not fully understood. The purpose of this study was to define the
source of milk Hp from dairy cows with naturally occurring mastitis. Quarter milk samples selected
from 50 dairy cows were separated into four groups according to SCC as group A: < 100 (n = 19);
B: 100–200 (n = 10); C: 201–500 (n = 10); and D: > 500 · 103 (n = 11) cells/mL. Our results
reveal that milk Hp concentrations were correlated with SCC (r = 0.742; P < 0.01), and
concentrations in group D were 10-fold higher than in group A. Reverse transcriptase-polymerase
chain reaction (RT-PCR) analysis indicates that the milk somatic cells from group D were not only
capable of synthesizing Hp but could also markedly increase Hp mRNA expression. Western blot,
immunocytochemistry, double confocal immunofluorescence, and Hp releasing experiments
demonstrate that neutrophils were associated with the biosynthesis and release of Hp in milk. It
further shows that Hp was significantly elevated in the epithelium of mammary gland tissue with
mastitis and was also expressed in the cultured mammary epithelial cells. We propose that
neutrophils and epithelial cells may play an essential role in elevating milk Hp in addition to previous
suggestions that Hp may be derived from mammary tissues and circulation

期刊名稱Vet. Res
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