Study of cryopreservation of articular chondrocytes using the Taguchi method | Faculty Open Information System

Study of cryopreservation of articular chondrocytes using the Taguchi method

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Study of cryopreservation of articular chondrocytes using the Taguchi method
出版類型SCI(Sciences Citation Index)
出版年度2010
AuthorsShaw-Ruey Lyu, 呂紹睿, Wei Te Wu(Benny) 吳瑋特, Chien Chih Hou 侯建志, & Wen-Hsin Hsieh 謝文馨
出版日期2010 / 4
其他編號0000
中文摘要

This study evaluates the effect of control factors on cryopreservation of articular cartilage chondrocytes
using the Taguchi method. Freeze–thaw experiments based on the L8(27) two-level orthogonal array of
the Taguchi method are conducted, and ANOVA (analysis of variables) is adopted to determine the statistically
significant control factors that affect the viability of the cell. Results show that the type of cryoprotectant,
freezing rate, thawing rate, and concentration of cryoprotectant (listed in the order of
influence) are the statistically significant control factors that affect the post-thaw viability. The end temperature
and durations of the first and second stages of freezing do not affect the post-thaw viability.
Within the ranges of the control factors studied in this work, the optimal test condition is found to be
a freezing rate of 0.61 ± 0.03 C/min, a thawing rate of 126.84 ± 5.57 C/min, Me2SO cryoprotectant,
and a cryoprotectant concentration of 10% (v/v) for maximum cell viability. In addition, this study also
explores the effect of cryopreservation on the expression of type II collagen using immunocytochemical
staining and digital image processing. The results show that the ability of cryopreserved chondrocytes to
express type II collagen is reduced within the first five days of monolayer culture.

期刊名稱Cryobiology
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