cDNA cloning, identification, tissue localization, and transcription profile of a transglutaminase from white shrimp, Litopenaeus vannamei, after infection by Vibrio alginolyticus.

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題cDNA cloning, identification, tissue localization, and transcription profile of a transglutaminase from white shrimp, Litopenaeus vannamei, after infection by Vibrio alginolyticus.
出版類型SCI(Sciences Citation Index)
出版年度2009
AuthorsMaw-Sheng Yeh, 葉茂盛, Chun-Hung Liu 劉俊宏, Yu-Chuan Huang 黃, & Winton Cheng 鄭文騰
開始頁748
頁數8
出版日期2009 / 1
其他編號0000
中文摘要

Complementary (c)DNA encoding transglutaminase (TG) messenger (m)RNA of white shrimp, Litopenaeus
vannamei, was cloned from haemocytes by a reverse-transcription polymerase chain reaction (RT-PCR)
and rapid amplification of cDNA ends (RACE) using oligonucleotide primers based on the TG sequence of
the horseshoe crab, Tachypleus tridentatus (accession no.: BAA02134); tiger shrimp, Penaeus monodon
(AAL78166); and Pacifastacus leniusculus (AF336805). The 2638-bp cDNA contained an open reading frame
(ORF) of 2172 bp, a 55-bp 50-untranslated region (UTR), and a 411-bp 30-UTR containing a poly A tail. The
molecular mass of the deduced amino acid (aa) sequence (757 aa) was 84.9 kDa with an estimated pI of 5.2.
The L. vannamei TG (abbreviated LvTG) contains a typical transglutaminase-like homologue, a putative
integrin-binding motif (RGD), and four calcium-binding sites; a catalytic triad is present as in arthropod
TG. Sequence comparison and phylogenetic analysis revealed that shrimp TG can be separated into two
subgroups, STGS1 and STGS2, and LvTG is more closely related to STGS1 than to STGS2. LvTG mRNA and
TG activities were detected in all tested tissues of L. vannamei, with LvTG mainly being synthesised in
haemocytes. However, the pattern of LvTG mRNA expression was not directly correlated with TG activity.
The haemocytes of L. vannamei injected with Vibrio alginolyticus showed a significant decrease of TG
activity at 3 h and a significant increase of LvTG mRNA expression at 6 h followed by a notable decrease
from 12 to 24 h, which indicated that cloned LvTG was involved in the immune response of shrimp. The
results also imply that more than one type of TG may be involved in the defense response in L. vannamei.

期刊名稱Fish Shellfish Immunol.
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