Identification and cloning of a selenium dependent glutathione peroxidase from giant freshwater prawn, Macrobrachium rosenbergii.

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Identification and cloning of a selenium dependent glutathione peroxidase from giant freshwater prawn, Macrobrachium rosenbergii.
出版類型SCI(Sciences Citation Index)
出版年度2009
AuthorsYen-hsiao Pan, 葉信平, Liu 劉冠甫, Chiu Shieh-Tsung 邱謝聰, 江順吉, Winton Cheng 鄭文騰, & Chun-Hung Liu 劉俊宏
開始頁181
頁數10
出版日期2009 / 1
其他編號NSC96-2313-B-020-008-MY3
中文摘要

A selenium dependent glutathione peroxidase (Se-GPx) cDNA was cloned from haemocyte by a reversetranscription
polymerase chain reaction (RT-PCR) and rapid amplification of cDNA (RACE). The 913 bp cDNA
contained an open reading frame (ORF) of 558 bp encoded a deduced amino acid sequence of 186 amino
acids. The prawn Se-GPx sequence contains a selenocysteine (Sec) residue which is encoded by the unusual
stop codon, 115TGA117. According to the molecular modeling analysis, the active site Sec residue, located in
the loop between b3 and a2 in a pocket on the protein surface, and hydrogen bonded to Gln73 and Trp141.
A GPx signature motif 2, 63LAFPCNQF70 and active site motif, 151WNFEKF156, two arginine (R) residues, R89
and R167 contribute to the electrostatic architecture that directs the glutathione donor substrate, and two
putative N-glycosylation site, 75NNT77 and 107NGS109 were observed in the prawn Se-GPx sequence. In
addition, the eukaryotic selenocysteine insertion sequence element is conserved in the 30-UTR. Comparison
of amino acid sequences showed that prawn Se-GPx is more closely related to vertebrate GPx 1. The prawn
Se-GPx was synthesized in haemocyte, hepatopancreas, muscle, stomach, gill, intestine, eyestalk, heart,
epidermis, lymph organ, ventral nerve cord, testis and ovary. The increase of respiratory burst in haemocyte
was observed in pathogen, Debaryomyces hansenii-injected prawn in order to kill the pathogen, and the
up-regulation in SOD and GPx acitivity, and prawn Se-GPx mRNA transcription were involved with the
protection against damage from oxidation.

期刊名稱Fish Shellfish Immunol.
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