Effects of arecoline on adipogenesis, lipolysis, and glucose uptake of adipocytes– a possible role of betel-quid chewing in metabolic syndrome

in Social Sciences Citation Index(SSCI), 社會科學引用文獻索引資料庫(SSCI)
標題Effects of arecoline on adipogenesis, lipolysis, and glucose uptake of adipocytes– a possible role of betel-quid chewing in metabolic syndrome
出版類型SSCI(Social Sciences Citation Index)
出版年度2010
AuthorsHsin-Fen Hsu, 許幸芬, Tsui-Chun Tsou 鄒粹軍, How-Ran Chao/Hao-Jan Chao 趙浩然, Cherng-Gueih Shy 施丞貴, Ya-Ting Kuo 郭晏廷, Feng-Yuan Tsai 蔡峰源, Szu-Ching Yeh 葉斯清, & Jing-Hua Lin 林晉華
開始頁370
頁數7
出版日期2010 / 6
其他編號0000
中文摘要

To investigate the possible involvement of betel-quid chewing in adipocyte dysfunction, we determined the effects of arecoline, a major alkaloid in areca nuts, on adipogenic differentiation (adipogenesis), lipolysis, and glucose uptake by fat cells. Using mouse 3T3-L1 preadipocytes, we showed that arecoline inhibited adipogenesis as determined by oil droplet formation and adipogenic marker gene expression. The effects of arecoline on lipolysis of differentiated 3T3-L1 adipocytes were determined by the glycerol release assay, indicating that arecoline induced lipolysis in an adenylyl cyclase-dependent manner. The diabetogenic effects of arecoline on differentiated 3T3-L1 adipocytes were evaluated by the glucose uptake assay, revealing that > or = 300 microM arecoline significantly attenuated insulin-induced glucose uptake; however, no marked effect on basal glucose uptake was detected. Moreover, using 94 subjects that were randomly selected from a health check-up, we determined the association of betel-quid chewing with hyperlipidemia and its related risk factors. Hyperlipidemia frequency and serum triglyceride levels of betel-quid chewers were significantly higher than those of non-betel-quid chewers. In this study, we demonstrated that arecoline inhibits adipogenic differentiation, induces adenylyl cyclase-dependent lipolysis, and interferes with insulin-induced glucose uptake. Arecoline-induced fat cell dysfunction may lead to hyperlipidemia and hyperglycemia/insulin-resistance. These findings provide the first in vitro evidence of betel-quid chewing modulation of adipose cell metabolism that could contribute to the explanation of the association of this habit with metabolic syndrome disorders.

期刊名稱Toxicology and Applied Pharmacology
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