Investigation of beta-glucans binding to human/mouse dectin-1 and associated immunomodulatory effects on two monocyte/macrophage cell lines.

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Investigation of beta-glucans binding to human/mouse dectin-1 and associated immunomodulatory effects on two monocyte/macrophage cell lines.
出版類型SCI(Sciences Citation Index)
出版年度2010
AuthorsMei-Li Wu, 吳美莉, Tzou-Chi Huang 黃卓治, Hung HJ H. H. J., Yu JH 余柔慧, chung 鍾文彬, & Hso-Chi Chaung 莊秀琪
開始頁1391
頁數8
出版日期2010 / 9
其他編號NSC97-2321-B-020-003
中文摘要

Dectin-1, a specific pattern recognition receptor for β-1,3/β-1,6-glucans, is expressed mainly on phagocytes. Human dectin-1 (hDectin-1) and mouse dectin-1 (mDectin-1) were separately expressed on HEK293 cell surfaces for examination of the binding abilities of a synthetic particulate β-glucan (pβG), a product extracted from Saccharomyces cerevisiae, in this study. The binding of zymosan-FITC to hDectin-1 and mDectin-1 was inhibited by pβG at similar concentrations for 50% inhibition of binding (IC50). However, the kinetics of the time course and dose response to zymosan stimulation observed for U937 and J774A.1 differed. Superoxide anion production was increased in U937 but reduced in J774A.1 when cells were treated with pβG, zymosan or laminarin, whereas ovalbumin endocytosis was enhanced in U937 and J774A.1 treated either with pβG, zymosan, laminarin or barley-glucan. These results indicate that the binding affinity of pβG to hDectin-1 is similar to the binding affinity to mDectin-1, and that stimulation by pβG as well as various forms of β-1,3-glucans on U937 and J774A.1 resulted in up-regulation of cell activity and ovalbumin endocytosis. Additionally, other co-receptors on U937 and J774A.1 may be involved in directing different responses to superoxide anion production in these two types of cells. These results will likely contribute to further investigations on identifying the biological forms of β-glucans capable of binding its specific receptor as the effective immunomodulators.

期刊名稱Biotechnol Prog
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