Shoot multiplication of Paphiopedilum orchid through in vitro cutting methods

in Domestic Journal (Other), 國內期刊(其他)
標題Shoot multiplication of Paphiopedilum orchid through in vitro cutting methods
Authors, W. U., P. - J. W. 2, Shih Wen Chin 金石文, & Fure-Chyi Chen 陳福旗
出版日期2012 / 12

Paphiopedilum orchids are generally propagated through the division of axillary buds from mother plant, which limits commercial production due to its unproductive proliferation and time consuming. In this study, the effect of in vitro cutting methods and medium composition on efficient shoot multiplication of Paphiopedilum Hsinying Rubyweb was investigated. Among three different in vitro stem cutting methods used, vertical cutting was able to produce more new shoots than horizontal and cross cutting when cultured on Hyponex based medium. After 12 weeks of culture, plantlets regenerated from vertical cutting were able to produce new healthy and well rooted shoots higher than without cutting on the same medium. Moreover, the newly-formed shoots which were divided into single plantlets and subcultured onto half-strength Murashige and Skoog (MS) medium without growth regulators could remain higher shoot multiplication than in other media. The micropropagation procedure developed in this study provides a simple means to in vitro propagate Paphiopedilum plantlets which are able to produce large numbers of uniform plantlets in a shorter time compared to the conventional propagation method.

期刊名稱African Journal of Biotechnology
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