Immunofluorescence Confocal Microscopy of Plant Cells

in International Symposium (oral presentation paper), 國際研討會(全文口頭發表)
標題Immunofluorescence Confocal Microscopy of Plant Cells
AuthorsJou YingTzy, 周映孜
會議名稱細胞分子影像暨螢光相關技術FRET, FLIM研討會
出版日期Dec 15 2008 12:0

Immunofluorescence labeled proteins in situ and useful to observe proteins localization in non-transformed plant cells. To avoid the plant auto-fluorescence interfered by chlorophyll, choosing the applicable spectrum of fluorescence secondary antibody is important, plant samples are recommend as callus, root tips or other low chlorophyll-containing tissue. Ice plant is a salt tolerance plant and not easy to transform. In order to understand a salt inducible protein mcSKD1 localization, five cellular marker part to double-labeled with mcSKD1 and identified position in ice plants. Confocal microscopy images display that mcSKD1 colocalization with the ER marker mEH and Golgi apparatus marker AtTLG2a. Two mcSKD1 interacting proteins mcSNF1 and mcCPN1 are ubiquitin-related, cell images presented high overlapping with mcSKD1. The arrangement data show mcSKD1 works on ER-Golgi to membrane ubiquitin-related movement. Immunofluorescence is not good for living cell study, but easy to supplement multiple proteins labeled on special species and fixable condition.

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