Characterization of newly emerging Newcastle disease viruses isolated during 2002-2008 in Taiwan.

in Social Sciences Citation Index(SSCI), 社會科學引用文獻索引資料庫(SSCI)
標題Characterization of newly emerging Newcastle disease viruses isolated during 2002-2008 in Taiwan.
出版類型SSCI(Social Sciences Citation Index)
出版年度2010
AuthorsGuan-Ming Ke, 柯冠銘, Hung Jen Liu 游信偉, 何志祥, 褚珮瑜, 柯良胤, Hung-Jen Liu 劉宏仁, & Hung-Jen Liu 林茂勇
開始頁247
頁數10
出版日期2010 / 2
其他編號0000
中文摘要

To elucidate the epidemiological relationships between ND outbreaks and genetic lineages, a portion of the F gene (535 bp) and the full-length HN gene (1922 bp) of recent Taiwanese NDVs isolated in 2002–2008 was amplified by reverse transcription (RT)-polymerase chain reaction (PCR) and sequenced. Only a portion of above amplified PCR products of the F andHNgenes (374 and 1713 bp) and their deduced amino acid residues were compared with the other 60 NDVs retrieved from GenBank. Most (29/30) of the recent Taiwanese isolates were clustered in subgenotype VIIe while only one isolate was classified as subgenotype VIIc. All the 29 isolates of subgenotype VIIe were further subclassified and termed provisionally as sub-subgenotypes VIIe2 (13 isolates), VIIe3 (5 isolates), and VIIe4 (11 isolates). The subsubgenotype VIIe2 isolates possessing the motif 112R-R-Q-K-R-F117 and amino acid residue substitutions at positions 23 (L to F) and 90 (T to A) were collected during 2002–2005. The sub-subgenotype VIIe3 isolates possessing the motif 112R-R-K-K-R-F117 and amino acid residue substitutions at positions 74 (E to G) and 75 (A to G) within epitopes and 114 (Q to K) within cleavage site of F protein were collected during
2003–2006. The sub-subgenotype VIIe4 isolates possessing the motif 112R-R-Q-K-R-F117 and amino acid residue substitutions at positions 23 (L to F), 26 (I to T), and 90 (T to A) were collected during 2007–2008. All the NDV isolates in this study exhibited a high intra-cerebral pathogenicity index (ICPI), they were all classified as velogenic type of NDVs. The sub-subgenotype VIIe2 and VIIe4 viruses are now dominant and have been implicatd in most of the recent ND outbreaks in Taiwan. Phylogenetic analysis of these isolates revealed that they may have evolved from previously reported local strains (VIIe1). This finding is essential for improving the disease control strategies and development of vaccines for ND.

期刊名稱Virus Res
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