Expression of plant ferredoxin-like protein (PFLP) enhances tolerance to heat stress in Arabidopsis thaliana

in International Symposium (poster summary), 國際研討會(摘要海報發表)
標題Expression of plant ferredoxin-like protein (PFLP) enhances tolerance to heat stress in Arabidopsis thaliana
出版類型國際研討會(摘要海報發表)
出版年度2013
AuthorsYi-Hsien Lin, 林宜賢
出版日期Jul 20 2013 12:0
會議地點Providence, RI
其他編號0000
中文摘要

Under adverse environments, plants produce reactive oxygen species (ROS), which can
trigger cell death when their accumulation surpasses the antioxidant capacity of ROS
scavenging systems. These systems function in chloroplasts mainly through the ascorbate-mediated water-water cycle, in which ascorbate is photoreduced by ferredoxin in the photosynthetic system. Our previous study showed that the fraction of the reduced form of ascorbate was increased in ferredoxintransgenic Arabidopsis (CPF) plants which overexpressed plant ferredoxin-like protein (PFLP) in their chloroplasts. Thus, we hypothesized that expression of PFLP could alter the tolerance of plants to abiotic stresses. In the present study, we found that two CPF lines exhibited lower mortality rates at five days, following two days of heat treatment. Compared to non-transgenic wild type (Col-0) plants, CPF plants exhibited decreased H2O2 content, MDA accumulation, and ion leakage after heat treatment. To confirm the efficacy of ferredoxin against heat stress in chloroplasts, we evaluated
two RNA interference (RNAi) lines of Atfd1 or Atfd2 Arabidopsis plants. Both lines exhibited adverse reactions following heat treatment. Based on these results, we conclude that overexpression of PFLP in chloroplasts can confer tolerance to heat stress in plants and that such an application of PFLP could help reduce plant mortality.

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