Development of a molecular method for rapid differentiation of watermelon lines resistant to Fusarium oxysporum f. sp. niveum

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Development of a molecular method for rapid differentiation of watermelon lines resistant to Fusarium oxysporum f. sp. niveum
出版類型SCI(Sciences Citation Index)
出版年度2009
AuthorsYing-Hong Lin, 林盈宏, Kan-Shu Chen 陳甘澍, Tsung-Dao Liou 劉政道, Jenn-Wen Huang 黃振文, & Pi-Fang Linda Chang 張碧芳
開始頁273
頁數7
出版日期2009 / 7
其他編號0000
中文摘要

The vascular wilt disease of watermelon caused by Fusarium oxysporum f. sp. niveum (Fon)
is one of the major factors limiting watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai) production worldwide. Breeding highly resistant lines is one of the best ways to control the disease. In order to accelerate the breeding programs, a reliable PCR (polymerase chain reaction) technique was developed to rapidly differentiate the disease-resistant watermelons. The G05-SCAR (sequence characterized amplified region) primer set GsF5/GsR5, derived from the OP-G05 random primer-amplified fragment sequence, produced an 898 bp DNA fragment which was specific to watermelon lines resistant or tolerant to Fon. With optimized PCR assay, the molecular method could easily differentiate Fon-resistant and -susceptible hybrid watermelon populations.

期刊名稱Botanical Studies
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