Development of a molecular marker for specific detection of Fusarium oxysporum f. sp. cubense race 4

in Social Sciences Citation Index(SSCI), 社會科學引用文獻索引資料庫(SSCI)
標題Development of a molecular marker for specific detection of Fusarium oxysporum f. sp. cubense race 4
出版類型SSCI(Social Sciences Citation Index)
出版年度2009
AuthorsYing-Hong Lin, 林盈宏, Jing-Yi Chang 張景宜, En-Tzu Liu 劉恩慈, Chih-Ping Chao 趙治平, Jenn-Wen Huang 黃振文, & Pi-Fang Linda Chang 張碧芳
開始頁353
頁數12
出版日期2009 / 3
其他編號0000
中文摘要

Fusarium oxysporum f. sp. cubense is the causal agent of Panama disease of banana. A rapid and reliable diagnosis is the foundation of integrated disease management practices in commodity crops. For this diagnostic purpose, we have developed a reliable molecular method to detect Foc race 4 isolates in Taiwan. By PCR amplification, the primer set Foc-1/Foc-2 derived from the sequence of a random primer OP-A02 amplified fragment produced a 242 bp size DNA fragment which was specific to Foc race 4. With the optimized PCR parameters, the molecular method was sensitive and could detect small quantities of Foc DNA as low as 10 pg in 50 to 2,000 ng host genomic DNA with high efficiency. We also demonstrated that by using our PCR assay with Foc-1/Foc-2 primer set, Foc race 4 could be easily distinguished from other Foc races 1 and 2, and separated other formae speciales of F. oxysporum.

期刊名稱European Journal of Plant Pathology
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