Protective function of the recombinant Oshsp18.0-II protein, a class II small heat shock protein of rice, in Escherichia coli.

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Protective function of the recombinant Oshsp18.0-II protein, a class II small heat shock protein of rice, in Escherichia coli.
出版類型SCI(Sciences Citation Index)
出版年度2012
AuthorsPi-Fang Linda Chang, 張碧芳, Wen-Kuan Huang 黃文寬, Ying-Hong Lin 林盈宏, Yuhsin Chen 陳裕星, & Tao-Ho Chang 張道禾
開始頁291
頁數8
出版日期2012 / 7
其他編號0000
中文摘要

All plants synthesize multiple families of small heat shock proteins (sHSP). A cDNA clone, Oshsp18.0-CII, encoding an 18.0 kDa class II sHSP was isolated from rice previously. The function of the rice class II sHSP was studied by overproduction of the Oshsp18.0-CII fusion protein in transformed Escherichia coli cells. The results suggest that heterologous expression of the Oshsp18.0-CII fusion protein increases thermotolerance of E. coli cells in vivo and provided thermoprotection to E. coli soluble proteins in vitro. The survival rate of Oshsp 18.0-CII fusion protein accumulating cells treated at 50°C for 1 h was almost 1000-fold higher than that of the control cells transformed with pET32a expression vector. Overproduction of the Oshsp18.0-CII fusion protein in E. coli also confers tolerance of E. coli cells to ultraviolet (UV) irradiation. The post-UV survival of the Oshsp18.0-CII fusion protein-accumulating cells is about 7.29-fold over that of the control cells transformed with pET32a expression vector when exposed to 1700 μJ of UV. There is almost no post-UV survival (≦0.4%) in the untransformed cells after exposing to 900 μJ UV light.

期刊名稱Botanical Studies
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