A molecular diagnosis method using real-time PCR for quantification and detection of Fusarium oxysporum f. sp. cubense race 4.

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題A molecular diagnosis method using real-time PCR for quantification and detection of Fusarium oxysporum f. sp. cubense race 4.
出版類型SCI(Sciences Citation Index)
出版年度2013
AuthorsYing-Hong Lin, 林盈宏, Ching-Chung Su 蘇慶昌, Chih-Ping Chao 趙治平, Chi-Yu Chen 陳啟予, Chung-Jan Chang 張宗仁, Jenn-Wen Huang 黃振文, & Pi-Fang Linda Chang 張碧芳
開始頁395
頁數10
出版日期2013 / 2
其他編號0000
中文摘要

The Fusarium genus causes devastating plant diseases worldwide, in which Fusarium oxysporum is the most serious crop pathogen. Disease monitoring is the basis of integrated pest management of any disease. The lack of rapid, accurate, and reliable device to detect and identify plant pathogens is one of the main limitations in integrated disease management. This study describes an efficient and quantifiable diagnosis method for the specific detection of F. oxysporum f. sp. cubense (Foc) race 4 in field-infected banana. With the optimized PCR parameters using the SCAR (sequence characterized amplified region) primers FocSc-1/FocSc-2 and a real-time PCR strategy, the developed method showed high reproducibility and was very sensitive to detect extremely low quantities of Foc genomic DNA (gDNA).We also found that Foc gDNA in severely symptomatic banana pseudostems and leaves were 6946-fold and 26.69-fold more than in those of mild-symptomatic banana, respectively.

期刊名稱European Journal of Plant Pathology
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