Effect of sucrose concentration and seed maturity on in vitro germination of Dendrobium nobile hybrids

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Effect of sucrose concentration and seed maturity on in vitro germination of Dendrobium nobile hybrids
出版類型SCI(Sciences Citation Index)
出版年度2013
AuthorsWaraporn Udomdee, 烏同地, Pei-Jung Wen 吳, Chen-Yu Lee 李鎮宇, Shih Wen Chin 金石文, & Fure-Chyi Chen 陳福旗
開始頁249
頁數6
出版日期2013 / 8
其他編號0000
中文摘要

Orchid is a major floral crop around the world and Dendrobium hybrids are considered to be one of the most popular orchids. In vitro germination of hybrid seeds is a common practice among orchid growers, however, in many cross pollinations the embryos may not develop to maturity, leading to poor seed germination. The effect of seed maturity and sucrose concentration were investigated via asymbiotic germination of nobile Dendrobium hybrids. Capsules were harvested from two hybrids (Den. Lucky Girl × Den. Second Love ‘Kirameki’ and Den. Lucky Girl × Den. Hamana Lake ‘Kumi’) and one selfing of Den. Second Love ‘Kirameki’ at 2, 3, 4, and 5 months after pollination and immature seeds were taken. Immature seeds from 3- to 5-month old capsules could be successfully germinated on Hyponex based medium. Immature seeds from 4-month old capsules showed greatest germination rate of tested treatments, whereas 3-month old immature seeds showed the least germination. After 6 weeks of in vitro culture, protocorms derived from embryos developed on every concentration of sucrose, but germination was greater at lower concentrations. Greater concentration of sucrose decreased normal-developed protocorms.

期刊名稱Plant Growth Regulation
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