Alternative splicing and genetic diversity of the white collar-1 (wc-1) gene in cereal Phaeosphaeria pathogens

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Alternative splicing and genetic diversity of the white collar-1 (wc-1) gene in cereal Phaeosphaeria pathogens
出版類型SCI(Sciences Citation Index)
AuthorsYing-Hong Lin, 林盈宏, Ericka Yen-Hsin Chiu 邱燕欣, Wei Wu W. W., Qijian Song Q. S., Pi-Fang Linda Chang 張碧芳, Ling-Yan Gao L. - Y. G., Chun-Chi Chou C. - C. C., & Peter P. Ueng P. P. U.
出版日期2010 / 7

The white collar-1 (wc-1) gene encodes an important light-responsive protein (wc-1) that maintains circadian clocks and controls numerous light-dependent reactions including sporulation in ascomycete fungi. The structure and expression of the wc-1 gene in wheatbiotype Phaeosphaeria nodorum (PN-w) was studied. It was shown that the full-size (3,353 bp in length) wc-1 gene in PN-w contained 4 introns in which introns 1 and 2 were flanked by GC-AG splice borders and were spliced constitutively. However, introns 3 and 4 of the wc-1 gene were alternatively spliced. As the result of alternative splicing (AS), six transcript variants were identified, encoding different lengths of deduced polypeptides(from 1,044 to 1,065aa). Ratios of the wc-1 gene transcript variants in the RNA population were the same in the sporulated and non-sporulated PN-w isolate Sn37-1 and the sporulated PN-w isolate S-79-1, grown under light/dark conditions. The AS of the wc-1 gene may control various light-dependent reactions in PN-w, which leads to diverse morphological, physiological and pathological characters for pathogen infection and spread. Based on the nucleotide and deduced amino acid sequences, the wc-1 gene in cereal Phaeosphaeria pathogens was diverse. It appeared that the deduced wc-1 polypeptide sequences of P. avenaria f. sp. avenaria (Paa), P. avenaria f. sp. triticea (Pat1 and Pat3) and barley biotype P. nodorum (PN-b) were more closely related than PN-w and Phaeosphaeeria sp. (P-rye) from Poland. Based on the wc-1 deduced polypeptide sequences, P. avenaria f. sp. triticea (Pat2) from foxtail barley (Hordeum jubatum L.) was evolutionary well separated from the other cereal Phaeosphaeria pathogens.

期刊名稱European Journal of Plant Pathology
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