Erythropoietin receptor signaling regulates both erythropoiesis and megakaryopoiesis in vivo

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Erythropoietin receptor signaling regulates both erythropoiesis and megakaryopoiesis in vivo
出版類型SCI(Sciences Citation Index)
AuthorsForgacova Katarina, none, Emanuel Necas 無, Ludek Sefc 無英文, & Ko-Tung Chang 張格東
出版日期2010 / 1

Transgenic expression of a gain-of-function truncated mouse erythropoietin receptor gene (EpoR) leads to
expansion of the HSC pool in response to human erythropoietin (Epo). We have re-examined this
observation using a knock-in mouse model, wherein the mouse EpoR gene was replaced in its proper genetic
locus by a single copy of either a wild-type human or a polycythemia-inducing truncated human EPOR gene.
Bone marrow cells obtained from knock-in mice were transplanted together with competitor bone marrow
cells in a model that allows tracking of erythroid, platelet, and leukocyte contributions by each genotype.
Secondary transplants were also performed. Stem/progenitor cells were identified phenotypically and
isolated for colony-forming assays to evaluate cytokine responsiveness by cells with the wild-type human or
truncated human EPOR gene. Augmented Epo signaling increased erythroid repopulation post-transplant as
expected, but had no effect on short-term or long-term leukocyte repopulation. However, the wild-type
human EPOR knock-in mouse showed decreases in both erythroid and platelet repopulation compared to
marrow cells from the mutant human EPOR knock-in mouse or normal B6 animals. These results provide
evidence supporting a role for Epo signaling in megakaryopoiesis in vivo and suggest a role for Epo signaling
early in hematopoietic development.

期刊名稱Blood Cells, Molecules, and Diseases
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