Evaluation on a Streptococcus suis Vaccine Using Recombinant Sao-L Protein Manufactured by Bioreactors as the Antigen in Pigs.

in Social Sciences Citation Index(SSCI), 社會科學引用文獻索引資料庫(SSCI)
標題Evaluation on a Streptococcus suis Vaccine Using Recombinant Sao-L Protein Manufactured by Bioreactors as the Antigen in Pigs.
出版類型SSCI(Social Sciences Citation Index)
出版年度2013
AuthorsChun-Yen Chu, 朱純燕, & Jai-Wei Lee 李嘉偉
出版日期2013 / 3
其他編號0000
中文摘要

Streptococcus suis (S. suis) can be classified into 33 serotypes based on the structure of capsular polysaccharides. Recent research indicated that a new surface protein designated as Sao (surface antigen one) reacts with 30 serotypes of convalescent-phase sera during S. suis infections, which makes Sao a good potential
antigen for developing S. suis vaccines. The objectives of this study were to produce recombinant Sao-L protein (rSao-L) from a strain of S. suis serotype 2 by a prokaryotic expression system in bioreactors and to use rSao-L as the antigen for a S. suis vaccine in mouse and swine models. The antibody titres in mice and pigs immunized with rSao-L were significantly (P < 0.05) increased. After challenge with live S. suis serotype 1 bacteria, the anatomical lesions in pigs immunized with rSao-L were reduced by 60%. These data indicated that immunization with rSao-L can confer cross-serotype protection against S. suis. Moreover, percentages of CD8+ and CD4+/CD8+ double-positive T cells in immunized pigs were significantly higher than those of the control group (P < 0.01). Using bioreactors to produce rSao-L as the antigen for S. suis vaccines may broaden protective efficacy and reduce production costs.

期刊名稱Transboundary and Emerging Diseases
校址:912 屏東縣內埔鄉學府路1號 總機:886-8-7703202 傳真:886-8-7740165 系統開發統維護單位:國立屏東科技大學 電算中心 版權所有