Micropropagation of self-heading Philodendron via direct shoot regeneration

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Micropropagation of self-heading Philodendron via direct shoot regeneration
出版類型SCI(Sciences Citation Index)
AuthorsFure-Chyi Chen, 陳福旗, & Jong-Yi Fang 方中宜
出版日期2015 / 1

The present study describes a direct shoot regeneration-based micropropagation procedure for the selfheading
cultivars of Philodendron. Three types of explant (i.e. leaf lamina, petiole, stem nodal segment)
were screened for their shoot induction potential following a three months treatment with 0.5 mg l?1 of
either 2,4-dichlorophenoxyacetic acid (2,4-D), thidiazuron (TDZ) or both. Results indicated that the leaf
laminas were poor candidates for shoot induction whereas the petioles showed potential for adventitious
shoot production at frequencies of 2.8–11.1% in two of the cultivars tested. Stem nodal segments were the
most responsive among the three as shoots formed directly following the TDZ treatment at frequencies
of 16.7–41.7% depending on the cultivar. When comparing the effectiveness of different cytokinins to
induce shoot proliferation on stem nodal segments, it was found that the 0.5 and 1 mg l?1 of kinetin (Kn)
and 6-benzyladenine (BA) treatments resulted in higher shoot formation percentages compared to the
0.5 and 1 mg l?1 of TDZ treatments in two of the three cultivars. Furthermore, more shoots were produced
on BA than on Kn-supplemented media in all the three cultivars. Shoots derived from the 0.5 mg l?1 of
BA treatment can be induced to root following one month incubation with 0.1–1 mg l?1 of indole-3-
butyric-acid (IBA). The rooted shoots showed 100% survival after acclimatization in the greenhouse. The
procedure reported in the present study can assist in the large-scale multiplication of elite self-heading
cultivars of Philodendron in the future.

期刊名稱Scientia Horticulturae
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