Cloning of Phytoene Desaturase Gene in Oncidium Gower Ramsey- Orchidaceae and Its Expression Analysis at Different Flower Stages

in English journal, 英文期刊
標題Cloning of Phytoene Desaturase Gene in Oncidium Gower Ramsey- Orchidaceae and Its Expression Analysis at Different Flower Stages
出版類型英文期刊(EI)
出版年度2012
AuthorsFure-Chyi Chen, 陳福旗
開始頁21
頁數15
出版日期2012 / 3
其他編號0000
中文摘要

Oncidium Gower Ramsey is an important ornamental orchid in Taiwan. The bright
yellow floral color is due to carotenoid accumulation in the flower tissues. In order to
understand the expression of phytoene desaturase (PDS), a key enzyme for the
biosynthesis of β-carotene, the cDNA encoding PDS was cloned and characterized
during flower development. The full length OgrPDS cDNA contains 2,273 bp, including
1,593 bp open reading frame encoding for a polypeptide of 531 amino acid residues. The
5’ and 3’ untranslated regions (UTR) were 293 and 387 bp, respectively. The deduced
amino acid sequence of this PDS has 72-75 % similarity to PDS from other plant species.
The relative expression level of the OgrPDS in roots and leaves was investigated. The
result showed that the expression level of OgrPDS at different flower developmental
stages increased gradually until stage 5 which two- and three- fold higher than leaves
and roots, respectively.

期刊名稱J. International Cooperation
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