Development and characterization of 16 polymorphic microsatellite markers from Taiwan cow-tail fir, Keteleeria davidiana var. formosana (Pinaceae) and cross-species amplification in other Keteleeria taxa

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Development and characterization of 16 polymorphic microsatellite markers from Taiwan cow-tail fir, Keteleeria davidiana var. formosana (Pinaceae) and cross-species amplification in other Keteleeria taxa
出版類型SCI(Sciences Citation Index)
出版年度2014
AuthorsChin-Shang Ho, 何錦尚, Huei-Chuan Shih H. - C. S. H. I. H., Ho-Yih Liu H. - Y. L., Shau-Ting Chiu S. - T. C., Mei-Hui Chen M. - H. C., Li-Ping Ju L. - P. J., Ya-Zhu Ko 郭雅筑, Chaur-Tzuhn Chen 陳朝圳, Yu-Shen Shih Y. - S. S., Tsai-Wen Hsu 蘇再文, & Yu-Chung Chiang 江友宗
開始頁2
頁數5
出版日期2014 / 4
其他編號0000
中文摘要

Background: Keteleeria davidiana var. formosana (Pinaceae), Taiwan cow-tail fir, is an endangered species listed on the IUCN Red List of Threatened Species and only two populations remain, both on the Taiwan Island. Sixteen polymorphic microsatellite loci were developed in an endangered and endemic gymnosperm species, Keteleeria
davidiana var. formosana, and were tested in an additional 6 taxa, K. davidiana var. calcarea, K. davidiana var. chienpeii, K. evelyniana, K. fortunei, K. fortunei var. cyclolepis, and K. pubescens, to evaluate the genetic variation available for conservation management and to reconstruct the phylogeographic patterns of this ancient lineage.
Findings: Polymorphic primer sets were developed from K. davidiana var. formosana using the modified AFLP and magnetic bead enrichment method. The number of alleles ranged from 3 to 16, with the observed heterozygosity ranging from 0.28 to 1.00. All of the loci were found to be interspecifically amplifiable.
Conclusions: These polymorphic and transferable loci will be potentially useful for future studies that will focus on identifying distinct evolutionary units within species and establishing the phylogeographic patterns and the process of speciation among closely related species.
Keywords: Keteleeria, Conservation, Microsatellites, Simple sequence repeat markers, Pinaceae

期刊名稱BMC Research Notes
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