Development of Species-Specific Primers for Agronomical Thrips and Multiplex Assay for Quarantine Identification of Western Flower Thrips.

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Development of Species-Specific Primers for Agronomical Thrips and Multiplex Assay for Quarantine Identification of Western Flower Thrips.
出版類型SCI(Sciences Citation Index)
出版年度2014
AuthorsNiann Tai Chang, 張念台
開始頁1728
頁數7
出版日期2014 / 10
其他編號?A09700939
中文摘要

While morphological identiTcation of thrips species has been difTcult because of their minute size and a lack of easily recognizable characteristics, molecular identiTcation based on the development of speciTc molecular markers can be easily and reliably carried out. Among the known molecular markers, the nuclear internal transcribed spacer (ITS) exhibits distinguishable variations among thrips species. In this study, sequences of ITS2 region of 10 agriculturally important thrips were established to design species-speciTc primers for polymerase chain reaction (PCR). ITS2 sequence variations within these species were far less than those among species, indicating the suitability of this marker for species-speciTc primers design. These primers, though with one or two sporadically variable positions, showed a good efTcacy within species. The speciTcity of these primers, examined on thrips species belonging to 15 genera, proved satisfactory. Furthermore, a multiplex PCR was used successfully for identifying Frankliniella occidentalis (Pergande), an insect pest monitored for quarantine purpose, and three additional thrips also commonly found in imported agricultural products and Teld samples, i.e., Thrips tabaci Lindeman, Thrips hawaiiensis (Morgan), and Frankliniella intonsa (Trybom). This study has demonstrated that speciTc primers and multiplex PCR based on ITS2 are reliable, convenient, and diagnostic tool to discriminate thrips species of quarantine and agricultural importance.

期刊名稱Journal of Economic Entomology
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