Na+ but not Cl- or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Na+ but not Cl- or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings
出版類型SCI(Sciences Citation Index)
出版年度2009
AuthorsChwan?Yang Hong, 洪傳揚, Yun-Yang Chao 趙雲洋, Min-YuYang 楊民宇, Shih-Chueh Cho 卓仕玨, & Ching-Huei Kao 高景輝
開始頁1598
頁數8
出版日期2009 / 4
其他編號0000
中文摘要

Glutathione reductase(GR,EC1,6.4.2)is an important reactive oxygen species-scavenging enzyme.The present study examined the relative importance of Na+,Cl, and the osmotic component in NaCl-induced expression of Oryza sativa glutathione reductase(OsGR) genes in rice roots.Semi-quantitative RT-PCR was used to quantify the mRNA levels for one cytosolic (OsGR2) and two chloroplastic (OsGR1 and OsGR3) isoforms of GR identified in the rice genome.The expression of OsGR2 and OsGR3 but not OsGR1 was increased in rice roots treated with NaCl.Treatment with 150 mM NaCl and 150 mM NaNO3 affected OsGR2 and OsGR3 induction similarly, which suggests that Na+ but notCl is required for the NaCl-induced expression of OsGR2 and OsGR3. We also show that Na+ but notCl is required for NaCl-enhanced GR activity and hydrogen peroxide(H2O2) production in rice roots.Inaddition to its component of ion toxicity, salt concentration in soil results in an osmotic effect.Here, we show that OsGR2 and OsGR3 expression, GR activity,and H2O2 content were
not affected at a concentration of mannitol iso-osmotic with 150 mM NaCl. NaCl-induced OsGR2 and OsGR3 in rice roots could be associated with Na+ but not an osmotic component.

網址http://www.sciencedirect.com/science/article/pii/S0176161709001333
期刊名稱 J Plant Physiol
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