Cytosine-phosphate-guanine oligodeoxynucleotides containing ACGTT motifs enhance the immune responses elicited by keyhole limpet hemocyanin antigen in dairy cattle.

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Cytosine-phosphate-guanine oligodeoxynucleotides containing ACGTT motifs enhance the immune responses elicited by keyhole limpet hemocyanin antigen in dairy cattle.
出版類型SCI(Sciences Citation Index)
出版年度2011
AuthorsShyh-Shyan Liu, 劉世賢, & Jai-Wei Lee 李嘉偉
開始頁323
頁數9
出版日期2011 / 7
其他編號0000
中文摘要

Adjuvants are important components of vaccine formulations. Effective adjuvants line innate and adaptive immunity by signaling through pathogen recognition receptors. Synthetic cytosine-phosphate-guanine (CpG)oligodeoxynucleotides (ODNs) have been shown to have potentials as adjuvants for vaccines. However, the immunostimulatory effect of CpG is species-specific and depends on the sequence of CpG motifs. A CpG ODN (2135), containing 3 identical copies of GTCGTT motif, was previously reported to have the strongest effects on bovine peripheral blood mononuclear cells (PBMC). Based on the sequence of 2135, we replaced the GTCGTT motif with 11 other sequences containing CG and investigated their effects on bovine lymphocyte proliferation.
Results showed that the CpG ODNs containing 3 copies of GACGTT motif had the highest lymphocyte stimulation index (7.91 – 1.18), which was significantly (P < 0.05) higher than that of 2135 (4.25 – 0.56). The CpG ODNs containing 3 copies of GACGTT motif also significantly increased the mRNA expression of interferon (IFN)-a, interleukin (IL)-12, and IL-21 in bovine PBMC. When dairy cows were immunized with the keyhole limpet hemocyanin (KLH) antigen formulated with CpG ODNs containing 3 copies of GACGTT, production of KLH-specific antibodies in serum and in milk whey was significantly (P < 0.05) enhanced. IFN-c in whole blood stimulated by KLH was also significantly (P < 0.05) increased in cows immunized with KLH plus CpG ODNs.
Our results indicate that CpG ODNs containing 3 copies of the GACGTT motifs is a potential adjuvant for bovine vaccines.

期刊名稱Nucleic acid therapeutics
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