A proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells.

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題A proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells.
出版類型SCI(Sciences Citation Index)
出版年度2014
AuthorsCheng Liu, 劉澄, Jen-Jie Lin 林振頡, Zih-Yan Yang 楊子研, Chi-Chu Tsai 蔡奇助, Liao Ming-Huei 徐睿良, & Yu-Jen Wu 吳裕仁
開始頁11672
頁數8
出版日期2014 / 12
其他編號NSC102-2113-M-020-001-MY2
中文摘要

Gallic acid (GA) has long been associated with a wide range of biological activities. In this study, its antitumor
effect against B16F10 melanoma cells was demonstrated by MTT assay, cell migration assay, wound-healing assay, and flow
cytometric analysis. GA with a concentration >200 μM shows apoptotic activity toward B16F10 cells. According to Western
blotting data, overexpressions of cleaved forms of caspase-9, caspase-3, and PARP-1 and pro-apoptotic Bax and Bad, accompanied
by underexpressed anti-apoptotic Bcl-2 and Bcl-xL indicate that GA induces B16F10 cell apoptosis via mitochondrial pathway.
The 2-DE based comparative proteomics was further employed in B16F10 cells with and without GA treatment for a large-scale
protein expression profiling. A total of 41 differential protein spots were quantified, and their identities were characterized using
LC-MS/MS analysis and database matching. In addition to some regulated proteins that were associated with apoptosis,
interestingly, some identified proteins involved in glycolysis such as glucokinase, α-enolase, aldolase, pyruvate kinase, and
GAPDH were simultaneously up-regulated, which reveals that the GA-induced cellular apoptosis in B16 melanoma cells is
associated with metabolic glycolysis.

網址http://pubs.acs.org/doi/abs/10.1021/jf504035s
期刊名稱Journal of Agricultural and Food Chemistry
校址:912 屏東縣內埔鄉學府路1號 總機:886-8-7703202 傳真:886-8-7740165 系統開發統維護單位:國立屏東科技大學 電算中心 版權所有