Isolation and characterization of a novel ACE-inhibitory tripeptide from enzymatic hydrolysis of soft-shelled turtle (Pelodiscus sinensis) egg white: an in-vitro, in-vivo and in-silico study

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Isolation and characterization of a novel ACE-inhibitory tripeptide from enzymatic hydrolysis of soft-shelled turtle (Pelodiscus sinensis) egg white: an in-vitro, in-vivo and in-silico study
出版類型SCI(Sciences Citation Index)
出版年度2014
AuthorsReynetha D.S. Rawendra, 藍文卓, Aisha 何艾莎, Sin-Hong Chen 陳信宏, Chang 張誌益, Wen-Ling Shih 施玟玲, Tzou-Chi Huang 黃卓治, Liao Ming-Huei 廖明輝, & Liao Ming-Huei 徐睿良
開始頁12178
頁數7
出版日期2014 / 12
其他編號NSC102-2113-M-020-001-MY2
中文摘要

In this study, a novel angiotensin-converting enzyme (ACE)-inhibitory tripeptide (IVR) was isolated and identified
from unfertilized soft-shelled turtle egg white (SSTEW). The IC50 value of IVR was measured in vitro as low as 0.81 ± 0.03 μM,
and its inhibition type was suggested as competitive according to the Lineweaver?Burk plot. This peptide can be generated from
either thermolysin followed by trypsin digestion (two stages) or only trypsin digestion (one stage). Quantitative LC?MS/MS analysis
indicated that two-stage digestion gave 3.14 ± 0.17 mg of IVR from 1 g of SSTEW, better than that from one-stage digestion (1.31 ±
0.12 mg). In vivo antihypertensive activity of the tripeptide IVR after single oral administration (0.1 and 1 mg/kg of body weight) led
to a significant reduction in systolic blood pressure 2?4 h after administration in spontaneously hypertensive rats. In addition, the
binding mechanism of IVR has been rationalized through docking simulations using the testicular ACE (tACE)?lisinopril complex
at 2 A resolution (PDB 108A). The best docking pose was located at the tACE catalytic site resembling the mode of inhibition exerted
by lisinopril, an effective hypertensive synthetic drug. The degree of inhibition of this peptide correlated with the H-bond interaction
between the C-terminal of IVR and Lys511 and Tyr520 residues of tACE, a significant inhibitor registration for lisinopril. This study
illustrated that IVR behaves as a transition-state analogue inhibitor and is useful in therapeutic intervention for blood pressure control.
To the best of our knowledge, this is the first report of an efficient ACE-inhibitory tripeptide generated from the unfertilized egg of
soft-shelled turtle.

網址http://pubs.acs.org/doi/abs/10.1021/jf504734g
期刊名稱Journal of Agricultural and Food Chemistry
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