Functional proteomic approach to discover geographic variations of king cobra venoms from Southeast Asia and China

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Functional proteomic approach to discover geographic variations of king cobra venoms from Southeast Asia and China
出版類型SCI(Sciences Citation Index)
AuthorsHui-Ching Chang, 張惠晴, Tein-Shun Tsai 蔡添順, & Inn-Ho Tsai 蔡蔭和
出版日期2013 / 6

This study deciphers the geographic variations of king cobra (Ophiophagus hannah) venom
using functional proteomics. Pooled samples of king cobra venom (abbreviated as Ohv)
were obtained from Indonesia, Malaysia, Thailand, and two provinces of China, namely
Guangxi and Hainan. Using two animal models to test and compare the lethal effects, we
found that the Chinese Ohvs were more fatal to mice, while the Southeast Asian Ohvs were
more fatal to lizards (Eutropis multifasciata). Various phospholipases A2 (PLA2s), three-finger
toxins (3FTxs) and Kunitz-type inhibitors were purified from these Ohvs and compared.
Besides the two Chinese Ohv PLA2s with known sequences, eight novel PLA2s were identified
from the five Ohv samples and their antiplatelet activities were compared. While two 3FTxs
(namely oh-55 and oh-27) were common in all the Ohvs, different sets of 3FTx markers were
present in the Chinese and Southeast Asian Ohvs. All the Ohvs contain the Kunitz inhibitor,
OH-TCI, while only the Chinese Ohvs contain the inhibitor variant, Oh11-1. Relative to the
Chinese Ohvs which contained more phospholipases, the Southeast Asian Ohvs had higher
metalloproteinase, acetylcholine esterase, and alkaline phosphatase activities.
Biological significance
Remarkable variations in five king cobra geographic samples reveal fast evolution and
dynamic translational regulation of the venom which probably adapted to different prey
ecology as testified by the lethal tests on mice and lizards. Our results predict possible
variations of the king cobra envenoming to human and the importance of using local
antivenin for snakebite treatment.

期刊名稱Journal of Proteomics
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