Cloning and characterization of Trimeresurus gracilis venom phospholipases A2: comparison with Ovophis okinavensis venom and the systematic implications

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Cloning and characterization of Trimeresurus gracilis venom phospholipases A2: comparison with Ovophis okinavensis venom and the systematic implications
出版類型SCI(Sciences Citation Index)
出版年度2012
AuthorsInn-Ho Tsai, 蔡蔭和, Tein-Shun Tsai 蔡添順, Ying-Ming Wang 王英敏, Ming-Chung Tu 杜銘章, & Hui-Ching Chang 張惠晴
開始頁151
頁數6
出版日期2012 / 11
其他編號0000
中文摘要

This study focuses on the structural and functional characterizations of novel venom
phospholipases A2 (PLA2s) from Trimeresurus gracilis, an endemic Taiwanese pitviper. The
PLA2 cDNAs were cloned from venom glands and sequenced. The majority of the clones
encoded a Glu6-containing PLA2 (designated as Tgc-E6) whose deduced amino acid
sequence resembled those of other Crotalinae acidic PLA2s. Tgc-E6 was also purified and
constituted about 6% (w/w) of the total venom proteins. For human platelet rich plasma,
Tgc-E6 inhibited the ADP- and collagen-induced aggregation with an IC50 of 272 nM and
518 nM, respectively. Like Ovophis okinavensis venom, T. gracilis venom did not contain any
Lys49-PLA2s, although a cDNA encoding Lys49-PLA2 has been cloned from each of the
species. Their predicted protein sequences are 94% identical, and their pI values 8.3 are
lower than those of other Lys49-PLA2s, mainly due to the acidic substitutions within
positions 78–111, which are apparently more similar to those in Tgc-E6 than to those in
other Lys49-PLA2s. This unique structural feature of the venom PLA2s thus render evidence
for close phylogenetic relationship between both species. The structural variations in the
venom acidic PLA2s of the two species possibly have resulted from adaptation to different
prey ecology.

期刊名稱Toxicon
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