Mammalian Ste20-like protein kinase 3 induces a caspase-independent apoptotic pathway.

in Sciences Citation Index(SCI), 科學引文索引資料庫(SCI)
標題Mammalian Ste20-like protein kinase 3 induces a caspase-independent apoptotic pathway.
出版類型SCI(Sciences Citation Index)
出版年度2010
AuthorsChia-Ying Lin, 林佳穎, Wu Hung-Yi 吳弘毅, Pei-Ling Wang 王佩玲, & Chiun-Jye Yuan 袁俊傑
開始頁98
頁數7
出版日期2010 / 1
其他編號A10000087
中文摘要

In this study, it was shown that the mammalian sterile 20-like serine/threonine protein kinase 3 (Mst3) plays an essential role in the staurosporine-induced apoptosis of HeLa cells. The staurosporine-induced apoptosis was reduced by around 65% by the selective knockdown of Mst3 in stable clones, HeLa(siMst3). Although caspases were shown to be involved in the Mst3-mediated apoptosis, only 15–20% of staurosporine-induced apoptosis was suppressed by the caspase inhibitor, z-DEVD-fmk. Accordingly, Mst3 was proposed to trigger a caspase-independent apoptotic pathway in response to staurosporine. Interestingly, staurosporine greatly induced the mitochondrial membrane potential transition in HeLa cells, but had no effect in Hela(siMst3). The role of Mst3 in controlling the mitochondrial integrity was therefore proposed, presumably through the regulation of Bax. Furthermore, it was shown that staurosporine promoted the nuclear translocation of apoptosis-inducing factor and endonuclease G in HeLa cells. The nuclease activity associated with endonuclease G was also enhanced in response to staurosporine. However, both staurosporine-induced nuclear translocation of apoptosis-inducing factor and endonuclease G and the nuclease activity associated with endonuclease G were markedly reduced in Hela(siMst3). These results suggest that Mst3 may respond to staurosporine to trigger the caspase-independent apoptotic pathway by regulating the nuclear translocation of apoptosis-inducing factor and endonuclease G, and the nuclease activity associated with endonuclease G.

期刊名稱 The International Journal of Biochemistry & Cell Biology
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