Applications of transcriptome sequencing technology for analysis of molecular mechanisms of Fusarium wilt resistance

in International Symposium (poster summary), 國際研討會(摘要海報發表)
標題Applications of transcriptome sequencing technology for analysis of molecular mechanisms of Fusarium wilt resistance
出版類型國際研討會(摘要海報發表)
出版年度2015
AuthorsPi-Fang Linda Chang, 張碧芳, Ying-Hong Lin 林盈宏, Tao-Ho Chang 張道禾, Kan-Shu Chen 施淑蓮, Kan-Shu Chen 陳甘澍, & Jenn-Wen Huang 黃振文
出版日期May 14 2015 12:0
會議地點Taranto, Italy
其他編號0000
中文摘要

Fungal pathogens belonging to the genus Fusarium cause devastating plant diseases worldwide. Among them, F. oxysporum causes damage to many economically important crops. Well understanding of the resistance mechanisms is the core of integrated pest management of any disease. In this study, two watermelon lines, Sugar Baby (SB, susceptible) and JSB (resistant) were challenged with F. oxysporum f. sp. niveum (Fon) and the transcriptome profiling was compared to elucidate the potential resistance mechanism. The results of whole transcriptome sequencing revealed that 57 genes were up-regulated in the shoot bases of resistant line JSB. On the other hand, 456 genes were up-regulated in the shoot bases of SB watermelon challenged with Fon. There were 426 genes differentially expressed-including 12 up-regulated and 414 down-regulated genes-in the shoot bases of Fon-inoculated JSB and SB combination (JSB-I/SB-I). Real-time qPCR analyses revealed that two flavonoid synthesis related genes (flavanone 3-dioxygenase-like and flavonoid 3’-monooxygenase-like) were up-regulated in the resistant line 3 and 6 days post-inoculation. The genes coding for transcription factors (WRKY51-like and WRKY70), pathogenesis-related proteins (PR proteins, thaumatin-like and PAR-1a) and an ethylene biosynthetic enzyme (ACC oxidase) were also up-regulated in the JSB line 3 to 9 days post inoculation. According to the results of gene expression profiles in Fon-susceptible SB and Fon-resistant JSB shoot bases inoculated with Fon, flavonoid synthesis related genes were expressed to higher levels in JSB at the 3rd day (early phase) and the 6th day (mid-phase) after inoculation. Other genes, i.e. genes for transcription factors, PR proteins and ACC oxidase, were expressed to higher levels in JSB during the 3rd to 9th days after inoculation.

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